Generator

Part:BBa_K2012016:Design

Designed by: Wenqi Huang   Group: iGEM16_HZAU-China   (2016-10-06)


A weak CheZ generator with fusion GFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1362


Design Notes

the linker between CheZ and GFP is well concerned. Its length and sequence are optimized by using structure analysis.


Source

the CheZ coding sequence of this part is amplified from the wild type E.coli K12 MG1655 strain, the sequence is completely assistant with the sequence published on NCBI.

References