Generator
Part:BBa_K2012016:Design
Designed by: Wenqi Huang Group: iGEM16_HZAU-China (2016-10-06)
A weak CheZ generator with fusion GFP reporter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 715
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1362
Design Notes
the linker between CheZ and GFP is well concerned. Its length and sequence are optimized by using structure analysis.
Source
the CheZ coding sequence of this part is amplified from the wild type E.coli K12 MG1655 strain, the sequence is completely assistant with the sequence published on NCBI.